The one that I know is Aimsun, which is defined as a traffic modelling software that allows you to model anything from a single bus lane to an entire region. It's a professional tool and I'm pretty sure it can do what you need.
There's also an Aimsun page on Wikipedia:
Aimsun software is used by government agencies, municipalities,
universities and consultants worldwide for traffic engineering,
traffic simulation, transportation planning and emergency evacuation
studies. It is used to improve road infrastructure, reduce emissions,
cut congestion and design urban environments for vehicles and
It's not a free application but if you just need to get some ideas and "play" with it you should be able to request a free trial version.
Here are a few images of the software in action taken from the official website: